Journal: Acta pharmacologica Sinica

Article Title: Avasimibe exerts anticancer effects on human glioblastoma cells via inducing cell apoptosis and cell cycle arrest.

doi: 10.1038/s41401-020-0404-8

Figure Lengend Snippet: Fig. 6 Avasimibe induced cell cycle arrest in the U251 and U87 cells. a Western blot analysis showed that avasimibe induced cell cycle arrest in the G0/G1 phase by regulating the p53/p21/p27 pathway. b Western blot analysis showed that avasimibe arrested the cell cycle in the G2/M phase by regulating the p53/GADD45A and Aurora A/PLK1 pathways. c Schematic model for the mechanism of avasimibe in cell cycle arrest

Article Snippet: The primary antibodies against cleaved caspase-9, cleaved PARP, p21, p27, CDK2, cyclin E1, CDK4, cyclin D, and PLK1 were purchased from Cell Signaling Technology; antibodies against Bax, p53, Aurora A, CDK1, cyclin B1, and GAPDH were purchased from Proteintech.

Techniques: Western Blot

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Functional roles of 12 hub genes.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Functional Assay, DNA Synthesis, Activation Assay

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Survival analysis of the 12 hub genes in MED based on the GSE30074 database. (A) AURKA, (B) BUB1B, (C) CCNB1, (D) CCNB2, (E) CHEK1, (F) KIF11, (G) KIF20A, (H) MAD2L1, (I) MCM6, (J) NCAPG, (K) RFC4, (L) RRM2; P < 0.05 was considered statistically significant.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Survival analysis of AURKA and KIF20A based on the GSE85217 database. (A) AURKA, (B) KIF20A. P < 0.05 was considered statistically significant.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: The different expressions of AURKA and KIF20A in the three databases. (A) Expressions of AURKA in GSE39182, (B) Expressions of AURKA in GSE74195, (C) Expressions of AURKA in GSE86574, (D) Expressions of KIF20A in GSE39182, (E) Expressions of KIF20A in GSE74195, (F) Expressions of KIF20A in GSE86574.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: AURKA’s biological involvement in tumors. (A) AURKA expression in different tumors. (B) AURKA gene STRING interaction network preview of interacting proteins (showing top 20 STRING interactants). (C) Illustration of KEGG pathway analysis findings using the ClueGO plugin in Cytoscape. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Pathway and process enrichment analysis of AURKA and top 20 interacting proteins.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Immunohistochemistry analysis of AURKA and KIF20A expression in Medulloblastoma tissues from Tianjin Huanhu Hospital (normal brain, n = 4; MED, n = 10). Scale bar = 50μm. Below is a list of statistical quantitative analyses. Data are mean ± SD. ***P < 0.001, one-way ANOVA.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Immunohistochemistry, Expressing

Journal: Oncotarget

Article Title: miR-331-3p and Aurora Kinase inhibitor II co-treatment suppresses prostate cancer tumorigenesis and progression

doi: 10.18632/oncotarget.18664

Figure Lengend Snippet: (A) Varying concentrations of AKi-II were used to establish an EC 50 of the inhibitor in LNCaP and 22Rv1 PCa cells. (B) LNCaP PCa cells were treated with 10 μM AKi-II over 5 days and proliferation was measured via cell titre. (C) The effect pretreatment of LNCaP PCa cells with miR-331-3p has on the EC 50 concentration of Aki-II. (D) Effects on colony formation of LNCaP PCa cells between miR-NC/miR-331-3p treated cells vs miR-NC/miR-331-3p and AKi-II treated cells. (E) Effects of si-RALA treatment on colony formation between si-RALA treated vs si-RALA and AKi-II treated 22Rv1 PCa cells. *p<0.05; CI=0.95; n=3, Error bars = SD.

Article Snippet: Trichostatin A (TSA) (Cat #T-8552) and 5-aza-2’-deoxycytidine (AZA) (Cat #A-3656) were from Sigma and Aurora Kinase inhibitor II (AKi-II) was from Santa Cruz Biotech (Cat #SC-203827).

Techniques: Concentration Assay

Journal: Oncotarget

Article Title: miR-331-3p and Aurora Kinase inhibitor II co-treatment suppresses prostate cancer tumorigenesis and progression

doi: 10.18632/oncotarget.18664

Figure Lengend Snippet: (A) miR-NC and miR-331-3p (+/- AKi-II; 10μM) xenograft NSG mice were monitored over a 33 day period for tumor size and volume. (B) Coronal and axial images from Day 33 representative mice from miR-NC and miR-3313p (+/- AKi--II; 10μM) xenograft groups. Tumor area is highlighted by red dash circle. (C) End point Kaplan-Meier survival curve of 22Rv1 xenograft mice +/- miR-NC/ miR-331-3p /AKi-II; 10μM). Log-rank (Mantel-Cox) Test; ***p<0.0005. Logrank test for Trend p<0.0011.

Article Snippet: Trichostatin A (TSA) (Cat #T-8552) and 5-aza-2’-deoxycytidine (AZA) (Cat #A-3656) were from Sigma and Aurora Kinase inhibitor II (AKi-II) was from Santa Cruz Biotech (Cat #SC-203827).

Techniques: